河南师范大学学报(自然科学版)

2022, v.50;No.226(05) 78-86+172

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超灵敏检测血清中葡萄糖的中空介孔普鲁士蓝@葡萄糖氧化酶电化学发光传感器
Electrochemiluminescence biosensor for ultrasensitive determination of glucose based on HMPB@GOx

仉华;申聪聪;陈粤华;樊思敏;
Zhang Hua;Shen Congcong;Chen Yuehua;Fan Simin;School of Chemistry and Chemical Engineering, Henan Normal University;

摘要(Abstract):

葡萄糖作为主要供能物质在生物体新陈代谢过程中发挥重要作用.目前已报道的葡萄糖分析方法主要是基于生物酶或纳米模拟酶催化葡萄糖联合光、电、色等检测技术构建的,如比色法、分光光度法、电化学法、高效液相色谱法等,然而仪器操作、生物亲和性、灵敏度等不足限制其在实际血糖检测中的应用.采用经典金属有机框架材料中空介孔普鲁士蓝(Hollow mesoporous Prussian Blue, HMPB)负载葡萄糖氧化酶(GOx)制备HMPB@GOx复合纳米材料,结合鲁米诺电化学发光(Electrochemiluminescence, ECL)性能构建一种超灵敏葡萄糖ECL体系.测试结果表明,立方结构的HMPB比表面积大、氧化还原活性高,同时作为酶载体及H_2O_2模拟酶级联放大ECL信号,在其他电活性物质如抗坏血酸(AA),多巴胺(DA)和尿酸(UA)共存时仍对葡萄糖具有良好的选择性.基于上述优良性能,该ECL传感体系对葡萄糖的检测范围为3.3×10(-2)~167.0μmol/L,检测限低至2.1×10(-2)~167.0μmol/L,检测限低至2.1×10(-2)μmol/L,成功检测实际血清中的葡萄糖含量.因此,该HMPB@GOx电化学发光传感体系具有成为糖尿病患者血清中葡萄糖便携检测器件的潜力.
As the main energy supply material, glucose plays an important role in the metabolism of organisms. To date, a series of glucose analysis methods were reported, such as colorimetry, spectrophotometry, electrochemistry and high performance liquid chromatography, which mainly based on biological enzyme or nano-mimic enzyme and combined with optical, electrical and color detection technologies and so on. In this study, an ultrasensitive electrochemiluminescence(ECL) biosensor for glucose detection was prepared based on hollow mesoporous prussian blue(HMPB), a classical metal organic framework material loading with amount of glucose oxidase(HMPB@GOx). The analysis results show that the cubic HMPB has large specific surface area and high redox activity, which acts as a carrier for GOx and H_2O_2 mimic enzyme to cascade amplify the ECL signal. And it still has good selectivity for glucose even coexisting with other electroactive substances such as ascorbic acid(AA), dopamine(DA) and uric acid(UA). Based on the above excellent performance, the ECL biosensor has a detection range of 3.3×10(-2)μmol/L,成功检测实际血清中的葡萄糖含量.因此,该HMPB@GOx电化学发光传感体系具有成为糖尿病患者血清中葡萄糖便携检测器件的潜力.
As the main energy supply material, glucose plays an important role in the metabolism of organisms. To date, a series of glucose analysis methods were reported, such as colorimetry, spectrophotometry, electrochemistry and high performance liquid chromatography, which mainly based on biological enzyme or nano-mimic enzyme and combined with optical, electrical and color detection technologies and so on. In this study, an ultrasensitive electrochemiluminescence(ECL) biosensor for glucose detection was prepared based on hollow mesoporous prussian blue(HMPB), a classical metal organic framework material loading with amount of glucose oxidase(HMPB@GOx). The analysis results show that the cubic HMPB has large specific surface area and high redox activity, which acts as a carrier for GOx and H_2O_2 mimic enzyme to cascade amplify the ECL signal. And it still has good selectivity for glucose even coexisting with other electroactive substances such as ascorbic acid(AA), dopamine(DA) and uric acid(UA). Based on the above excellent performance, the ECL biosensor has a detection range of 3.3×10(-2)-167.0 μmol/L for glucose, and the detection limit is as low as 2.1×10(-2)-167.0 μmol/L for glucose, and the detection limit is as low as 2.1×10(-2) μmol/L, which successfully detects the glucose in the actual serum. Therefore, the ECL biosensor based on HMPB@GOx has potential as a portable glucose detection device for diabetics.

关键词(KeyWords): 中空介孔普鲁士蓝(HMPB);电化学发光;鲁米诺;葡萄糖氧化酶(GOx);葡萄糖
hollow mesoporous prussian blue (HMPB);electrochemiluminescence;luminol;glucose oxidase(GOx);glucose

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(22004028;21722501;21803018);; 河南省重点科技攻关项目(202102310139;212102311071)

作者(Authors): 仉华;申聪聪;陈粤华;樊思敏;
Zhang Hua;Shen Congcong;Chen Yuehua;Fan Simin;School of Chemistry and Chemical Engineering, Henan Normal University;

DOI: 10.16366/j.cnki.1000-2367.2022.05.011

参考文献(References):

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