孙雨安;孙敏青;王国庆;柯玮;李振兴;于文浩;
SUN Yu'an;SUN Minqing;WANG Guoqing;KE Wei;LI Zhenxing;YU Wenhao;Department of Material and Chemical Engineering,Zhengzhou University of Light Industry;

• 1:郑州轻工业学院材料与化学工程学院

摘要(Abstract)：

不同品种蜂蜜经顶空-固相微萃取(HS-SPME),萃取得到的样品经气相色谱-质谱(GC-MS)分析确认得到蜂蜜中挥发成分种类.以糖浆作为蜂蜜模拟物,通过在其中添加不同量的苯甲醛建立HS-SPME GC-MS标准工作曲线.结果表明,定量模型苯甲醛的线性范围为0.022 4～0.224μg·g-1,r2=0.995 6,测定方法的相对标准偏差(RSD)为5.45%;采用质谱谱库检索结合文献资料定性确认7种不同品种蜂蜜挥发性成分,得到了135个组分信息,其中7种蜂蜜中共有的化合物仅有44种,其中主要成分脱氢芳樟醇的含量为6.86×10-3～1 222.7×10-3μg·g-1,不同品种的挥发成分及同一蜂蜜样品中的不同结构特征的挥发成分相对含量差别较大.
The volatile components in honey can be confirmed by analysis of samples extracted by HS-SPME from different honey through GC-MS.The standard curve of HS-SPME-GC/MS was established by adding different amount benzaldehyde to honey syrup as honey simulate.The results showed that the linear range of benzaldehyde was 0.022 4～0.224 0μg·g-1,r2=0.995 6,RSD=5.45%.135pieces of component information was gained by searching in mass spectral library and document literature to confirm volatile components of 7kinds of honey,the shared kinds of component in 7kinds of honey 44and the content of main component Hotrienol 6.86×10-3～1 222.7×10-3 μg·g-1.The relative amount of the volatile component in different honey and the volatile component of different structure in the same honey was different.

关键词(KeyWords)： 顶空固相微萃取;气相色谱-质谱;蜂蜜;挥发性成分
honey;volatile components;headspace solid-phase microextraction(HS-SPME);gas chromatography mass spectrometry(GC-MS)

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(21075113);; 河南省高校科技创新人才支持计划项目(2009HASTIT026)

作者(Authors): 孙雨安;孙敏青;王国庆;柯玮;李振兴;于文浩;
SUN Yu'an;SUN Minqing;WANG Guoqing;KE Wei;LI Zhenxing;YU Wenhao;Department of Material and Chemical Engineering,Zhengzhou University of Light Industry;

DOI: 10.16366/j.cnki.1000-2367.2013.05.010

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