河南师范大学学报(自然科学版)

2022, v.50;No.226(05) 124-133

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基于宏基因组学研究怀地黄对鲤鱼肠道微生物群落组成的影响
Metagenomic insights into the effects of dietary Rehmannia glutinosa on the intestinal microbial composition of common carp

常绪路;康美如;冯军厂;张建新;
Chang Xulu;Kang Meiru;Feng Junchang;Zhang Jianxin;College of Fisheries;Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation,Henan Normal University;

摘要(Abstract):

鲤鱼(Cyprinus carpio L.)是我国淡水养殖鱼类的主要品种之一,其养殖过程中病害给养殖业带来重大经济损失.已有研究表明怀地黄(Rehmannia glutinosa,RG)作为饲料添加剂,具有提高鲤鱼免疫力和抵抗病害的功能,但机制尚不明确.利用宏基因组技术研究饲料中添加4%(质量分数)怀地黄对鲤肠道微生物群落组成的影响.结果表明梭杆菌门(Fusobacteria),变形杆菌门(Proteobacteria),拟杆菌门(Bacteroidetes),广古菌门(Euryarchaeota),奇古菌门(Thaumarchaeota),短尾噬菌体(Siphoviridae),肌尾噬菌体(Myoviridae),长尾噬菌体(Podoviridae),子囊菌门(Ascomycota)和担子菌门(Basidiomycota)是鲤肠道主要微生物类群.怀地黄可以增加鲤鱼肠道中益生菌、短链脂肪酸产生菌和产甲烷古菌的丰度,同时降低一些鱼类病毒的丰度.研究结果表明,饲料中添加怀地黄可以改善鲤鱼肠道微生物组成,提高有益菌的丰度而降低有害微生物的含量.研究结果为解析怀地黄增强鲤鱼免疫力的机制奠定了基础.
The common carp(Cyprinus carpio L.) is the most widely farmed freshwater fish in China. However, the intensive rearing of this fish has generated a potential environmental stress, which leads to a high susceptibility of the fish to various disease agents. Therefore, various infectious diseases erupt and lead to huge economic losses in aquaculture. Previous studies have indicated that dietary supplementation with Rehmannia glutinosa(RG)preparations has the potential to promote growth performance, regulate immune response and prevent pathogenic infection in common carp. In this study, metagenomics approach was used to investigate the effects of 4% RG on the microbial community composition in the gut of common carp. The dominated microorganisms in the gut of common carp were Fusobacteria, Proteobacteria, Bacteroidetes, Euryarchaeota, Thaumarchaeota, Siphoviridae, Myoviridae, Podoviridae, Ascomycota and Basidiomycota. Compared with the common carp fed a control diet, the common carp fed an RG-supplemented diet contained a higher content of probiotics, short-chain fatty acid(SCFA)-producing bacteria and methanogenic archaea, but contained a lower content of fish viruses. The results of the study showed that diet with RG could improve intestinal microbes of common carp, thereby promoting the health of common carp. The results of this study lay the foundation for the release of the mechanism of RG improve the immunity of common carp.

关键词(KeyWords): 怀地黄;鲤鱼;肠道微生物组;宏基因组
Rehmannia glutinosa;common carp;intestinal microorganisms;metagenomic

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31902361)

作者(Authors): 常绪路;康美如;冯军厂;张建新;
Chang Xulu;Kang Meiru;Feng Junchang;Zhang Jianxin;College of Fisheries;Engineering Technology Research Center of Henan Province for Aquatic Animal Cultivation,Henan Normal University;

DOI: 10.16366/j.cnki.1000-2367.2022.05.017

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