王磊,陈军平,王娅,施文瑞,李学军,董丽,张笑谦
Wang Lei,Chen Junping,Wang Ya,Shi Wenrui,Li Xuejun,Dong Li,Zhang Xiaoqian

• 1:河南师范大学水产学院

摘要(Abstract)：

为了揭示河南省不同黄河鲤养殖群体之间的遗传差异,本研究通过线粒体COI基因对5个黄河鲤群体的遗传多样性进行了研究.5个群体中荥阳水产良种场群体的单倍型数为4,洛阳水产技术推广站群体的单倍型数为2,其它群体的单倍型数均为3;各个群体的基因多样性从0.200到0.750不等,最高的为河南省水产良种繁育场的群体;各群体的核苷酸多样性范围为0.000 63到0.002 28,最高的为荥阳水产良种场群体,最低的为洛阳市水产技术推广站群体.分子方差分析表明黄河鲤将近90%的变异来自于群体内,说明整体上群体间分化较小.结果表明,虽然各个黄河鲤群体之间的遗传分化较小,但是它们之间的遗传多样性差别较大,研究结果可以为黄河鲤的育种和增殖放流等工作提供一定的参考.
The mitochondrial COI gene of five Yellow River carp populations was sequenced to reveal their genetic diversity.In the five populations,the number of haplotype in the population of Xingyang Aquatic Breeding Farm and the population of Luoyang Aquatic Technology Promotion Station was 4 and 2 respectively.The other haplotype number was 3.Genetic diversity of the populations ranged from 0.200 to 0.750.The highest was the population of Henan Aquatic Breeding Farm.The population of Luoyang Aquatic Technology Promotion Station was lower than other populations.Nucleotide diversity of the populations was in the range of 0.000 63 to 0.002 28.The highest was the population of Xingyang Aquatic Breeding Farm and the lowest was the population of Luoyang Aquaculture Technology Promotion Station.Analysis of molecular variance shew that nearly90% of the variation came from the interior of the populations.The result indicated that although genetic differentiation was small between populations,but genetic diversity was relatively large in different populations.The study provided some basis for artificial releasing and breeding new varieties of the Yellow River carp.

关键词(KeyWords)： 黄河鲤;养殖群体;COI基因;遗传多样性
Cyprinus carpio haematopterus Temminck et Schlegel;cultured population;COI gene;genetic diversity

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31602149);; 国家级大学生创新训练项目(201710476019);; 河南省高等学校重点科研项目(17B240001;17A240002);; 河南师范大学博士科研启动基金(qd14184)

作者(Author): 王磊,陈军平,王娅,施文瑞,李学军,董丽,张笑谦
Wang Lei,Chen Junping,Wang Ya,Shi Wenrui,Li Xuejun,Dong Li,Zhang Xiaoqian

DOI: 10.16366/j.cnki.1000-2367.2018.03.015

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