河南师范大学学报(自然科学版)

2021, v.49;No.221(06) 47-54

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草地生态系统微生物群落对季节性水淹的响应
Responses of soil microbial community to seasonal flooding in a grassland ecosystem

刘啸林;闫鑫宇;刘珂彤;刘银占;赵灿灿;韩士杰;王松廷;刘梦舟;
Liu Xiaolin;Yan Xinyu;Liu Ketong;Liu Yinzhan;Zhao Cancan;Han Shijie;Wang Songting;Liu Mengzhou;School of Life Sciences,Henan University;School of Ecology and Environmental Sciences,East China Normal University;College of Geography and Environmental Science,Henan University;

摘要(Abstract):

为阐明季节性水淹对微生物群落结构及特征的影响,通过模拟长期季节性水淹实验,设置冬季水淹、全年水淹和非水淹3个处理,利用高通量测序技术对土壤细菌和真菌进行测序,并结合生物信息学分析土壤微生物群落组成对水淹的响应.结果表明,经过长期水淹处理下的优势细菌类群为变形菌门、放线菌门和酸杆菌门;优势真菌类群为子囊菌门和毛霉亚门.水淹处理下独享的细菌和真菌OTU数量均高于非水淹处理.全年水淹和冬季水淹处理下的真菌和细菌群落结构较非水淹处理具有显著性差异.水淹条件下,土壤湿度和土壤pH分别是调控细菌和真菌群落的最佳环境因子.季节性水淹可以通过调控土壤理化性质进而改变草地生态系统微生物群落组成和结构,且土壤细菌对季节性水淹的敏感程度高于真菌.
To test the effects of seasonal flooding on microbial community structure and characteristics in the grassland. In the study, a simulation experiments were used to set up three treatments for winter-flooding, annual-flooding and non-flooding(control). We use high-throughput sequencing technology to sequence the soil bacteria and fungi, and combining bioinformatics to analyze the composition of soil microbial communities how to response in flooding. The results showed that the dominant bacterial groups under long-term flooding treatment are Proteobacteria, Actinobacteria and Acidobacteria; the dominant fungal groups under long-term flooding treatment are Ascomycota and Mucoromycota. The number of bacteria and fungi OTU exclusive to the flooded treatment are higher than that of the control. The community structure of fungi and bacteria under the annual-flooding and winter-flooding treatments is significantly different than that of the control. Under flooded conditions, soil moisture and soil pH are the best environmental factors to regulate bacterial and fungal communities, respectively. All in all, seasonal flooding can change the composition and structure of the microbial community of the campus grassland ecosystem by regulating the physical and chemical properties of the soil, and the sensitivity of soil bacteria to seasonal flooding is higher than that of fungi.

关键词(KeyWords): 草地生态系统;降水改变;微生物群落;高通量测序
grassland ecosystem;changing precipitation;microbial community;high-throughput sequencing

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(41930643);; 河南省重大公益项目(201300311300);; 河南省科技攻关项目(192102110085);; 中国博士后科学基金(2018M642738;2018M642739)

作者(Author): 刘啸林;闫鑫宇;刘珂彤;刘银占;赵灿灿;韩士杰;王松廷;刘梦舟;
Liu Xiaolin;Yan Xinyu;Liu Ketong;Liu Yinzhan;Zhao Cancan;Han Shijie;Wang Songting;Liu Mengzhou;School of Life Sciences,Henan University;School of Ecology and Environmental Sciences,East China Normal University;College of Geography and Environmental Science,Henan University;

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DOI: 10.16366/j.cnki.1000-2367.2021.06.007

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